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3a10 mouse anti neuro lament associated protein antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank 3a10 mouse anti neuro lament associated protein antibody
    3a10 Mouse Anti Neuro Lament Associated Protein Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+neuro+lament/anti-neurofilament-associated/ppr0433703-62-23-30
    Average 95 stars, based on 85 article reviews
    3a10 mouse anti neuro lament associated protein antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Ephrin-A3 Is Required for Tonotopic Map Precision and Auditory Function in the Mouse Auditory Brainstem
    Article Snippet: For staining, explant cultures were xed with 4% PFA in 0.1M sodium cacodylate, pH 7.4 (11650, Electron Microscopy Sciences) containing 10 mM MgCl2 and 5 mM CaCl2 for 12 min and washed with 0.1M cacodylate buffer followed by PBS. .. Explants were blocked for one hour at room temperature in PBS containing 1% tween-20, 5% DMSO, 1% sh gelatin, and 1.4 mg/ml BSA (PBSTD, all from MilliporeSigma), followed by a 1-hour incubation in mouse anti-neuro lament (2H3, Developmental Studies Hybridoma Bank), mouse anti-GAP43 (MAB347, MilliporeSigma), and rabbit anti-Myosin-VI (25-6791, Proteus BioSciences). .. Explants were washed three times in PBSTD, incubated in Alexa Fluor-conjugated secondary antibodies at RT for 40 min, washed three times again in PBSTD, and mounted in Fluoromount-G. Confocal images were obtained on an Olympus FluoView FV1000 using a XL Fluor 4X/340 (NA:0.28) or a 10X (NA:0.40) objective, or a 60X (NA:1.40) oil-immersion objective.



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